parent strain trichoderma reesei tu 6 Search Results


jecorina  (ATCC)
93
ATCC jecorina
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Jecorina, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal tu 6 anti β tubulin  (Danaher Inc)
86
Danaher Inc mouse monoclonal tu 6 anti β tubulin
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Mouse Monoclonal Tu 6 Anti β Tubulin, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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6-propyl-2thiouracil (i’tu  (Millipore)
90
Millipore 6-propyl-2thiouracil (i’tu
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
6 Propyl 2thiouracil (I’tu, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kh2po4 tu 6-09-5324-87  (Chimmed Inc)
90
Chimmed Inc kh2po4 tu 6-09-5324-87
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Kh2po4 Tu 6 09 5324 87, supplied by Chimmed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tu6-11  (Millipore)
90
Millipore tu6-11
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Tu6 11, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antiacetylated (ac)-α tubulin (tu  (Millipore)
90
Millipore monoclonal antiacetylated (ac)-α tubulin (tu
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Monoclonal Antiacetylated (Ac) α Tubulin (Tu, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tu6 pytk095 addgene  (Addgene inc)
93
Addgene inc tu6 pytk095 addgene
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Tu6 Pytk095 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tu6 pytk067 addgene  (Addgene inc)
93
Addgene inc tu6 pytk067 addgene
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Tu6 Pytk067 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xtalab synergy r diffractometer  (Rigaku Corporation)
90
Rigaku Corporation xtalab synergy r diffractometer
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Xtalab Synergy R Diffractometer, supplied by Rigaku Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with the pyr4 gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.

Journal: BMC Biology

Article Title: Light-dependent roles of the G-protein α subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei )

doi: 10.1186/1741-7007-7-58

Figure Lengend Snippet: Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with the pyr4 gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.

Article Snippet: The uridine auxotrophic H. jecorina ( T. reesei ) strain TU-6 (ATCC MYA-256; Δ pyr4 ; [ ]) was used for construction of the recombinant strains described in this study and maintained on malt extract agar supplemented with 10 mM uridine (Sigma-Aldrich, Madison, WI, USA).

Techniques: Construct, Transformation Assay, Selection, Marker, Activation Assay, Cloning